外源硅调控葡萄生理特性对低温胁迫的响应

本研究以大田葡萄品种‘梅鹿辄’为试材,采用大田喷施硅酸钾与室内低温处理相结合的方法,测定不同温度下葡萄叶片生理指标的变化,研究外源硅酸钾调控葡萄生理特性对低温胁迫的响应,探讨其提高葡萄抗寒性可能的作用机制。结果表明:低温胁迫下葡萄Inv活性被抑制,而其它指标则升高;喷施硅酸钾可显著降低低温胁迫下葡萄叶片的MDA含量,且当硅酸钾浓度为0.75%时对低温的损伤缓解作用最大;而脯氨酸、可溶性蛋白和可溶性糖含量、Inv、SPS活性及SS净活性均随硅酸钾浓度的升高而显著提高,但是可溶性蛋白含量在硅酸钾浓度为0.50%时最大,其它指标均在浓度为0.75%时最大;通过葡萄叶片各抗寒指标的隶属度值与综合评价指标看出,始花期0.75%的硅酸钾处理后葡萄抗寒性最强。综合分析,喷施硅酸钾可提高低温胁迫下葡萄叶片渗透调节物质含量,加快蔗糖转运及代谢速率,缓解活性氧累积,增强其耐寒性。     

Density responses and spatial distribution of cotton yield and yield components in jujube (Zizyphus jujube)/cotton (Gossypium hirsutum) agroforestry

Trees are the dominant species in agroforestry systems, profoundly affecting the performance of understory crops. Proximity to trees is a key factor in crop performance, but rather little information is available on the spatial distribution of yield and yield components of crop species under the influence of trees in agroforestry systems. Also, little information is available on how crop density may be exploited to optimize the yield in such systems. Here we studied the performance of cotton in jujube/cotton agroforestry. Field experiments were conducted in 2012 and 2013 in Hetian, Xinjiang, China. Cotton was grown at a row distance of 60 cm in three densities, 13.5, 18.0 and 22.5 plants m⁻² in six m wide paths between tree lines in a jujube plantation. Plant density affected both cotton aboveground dry matter and yield significantly. The highest yield was attained at the intermediate density of 18.0 plants m⁻² (20.0 plants m⁻² corresponding in sole cotton), lower than the optimal density in sole cotton (25.0 plants m⁻²). Yield at the lower density was constrained by the low number of bolls per m² as a direct consequence of the low density, whereas at the high plant density yield was constrained by a lower allocation of assimilates to cotton seed and lint, as a consequence of intraspecific and interspecific competitions. There were strong gradients in yield and yield components in relation to the distance from the tree rows. Leaf area and total dry matter of cotton in rows close to the tree lines were reduced, especially in the rows next to the trees. Moreover, biomass allocation to cotton fruits was reduced in these rows. Competitive influences from the trees on cotton performance extended two rows deep in a six-year old jujube stand, and even three rows deep in a seven-year old stand. Shading effects on cotton yield were compensated by increasing plant density as a result of greater boll numbers per unit ground area. Data from this study help guide the design of optimal plant density of cotton in jujube plantations and give insight in the spatial distribution and dynamics of competitive effects in agroforestry systems in general.     

Atmospheric humidity and genotype are key determinants of the diurnal stomatal conductance pattern

Great understanding of the genotypic difference in diurnal stomatal conductance (g_s) pattern and the key determinants of the pattern is important for saving water by adopting cultivars appropriately. Fifteen wheat genotypes were studied under different soil conditions and various meteorological conditions with pot cultivation in rain shelter for two years. Genotype and air humidity were found to be key determinants of diurnal g_s pattern. All genotypes under low relative humidity (LRH) and most genotypes under high relative humidity (HRH) displayed a gradual decline pattern from morning through the afternoon. Under moderate relative humidity (MRH), all genotypes present a single-peak curve pattern, but they differed in peak time, which may lead to unreliable g_s comparison between genotypes and get ostensible contrasting materials. The stomatal conductance was significantly different among genotypes under LRH and the increased g_s magnitude is also significantly different when it was compared between LRH and HRH. The present results provide new thinking for selecting and adopting appropriate cultivars with specific stomata traits for the area with various meteorological conditions.     

Agro-hydrological evaluation of on-farm rainwater storage systems for supplemental irrigation in Laikipia district,Kenya

Semi-arid agro-ecosystems are characterized by erratic rainfall and high evaporation rates leading to unreliable agricultural production. Total seasonal rainfall may be enough to sustain crop production, but its distribution and occurrence of intra-season dry spells (ISDS) and off-season dry spells (ODS) affect crop production. Rainwater harvesting (RWH) and management, especially on-farm storage ponds for supplemental irrigation offers an opportunity to mitigate the recurrent dry spells. Farm ponds are small runoff storage structures of capacities ranging from 30 to 100 m³ used mainly for supplemental irrigation of kitchen gardens, and sometimes for domestic and livestock water supply. The main objective of the study was to evaluate the hydrological and economic performance of farm ponds with the view of assessing their contributions to water and food security in semi-arid agro-systems of Kenya. Agro-hydrological evaluation of on-farm runoff storage systems entailed field survey, monitoring of water losses, analysis of rainy seasons and dry spell occurrence, soil moisture and water balance, estimation of supplemental irrigation requirement (SIR) and farm-level cost-benefit analysis of cabbage production using low-head drip irrigation system. Significant water losses through seepage and evaporation, which accounted on average for 30–50% of the stored runoff, is one of the factors that affect the adoption and up-scaling of on-farm water storage systems. Frequency analysis of rainfall revealed that there is 80% probability of occurrence of dry spells exceeding 10 and 12 days during the long rains and short rains, respectively. The occurrence of off-season (after rainfall cessation) dry spells was more pronounced than intra-seasonal (within the rainy season) dry spells. The length of intra-seasonal (10–15 days) was less than off-season dry spells (20–30 days). The occurrence of off-season dry spells coincides with the critical crop growth stage, in particular flowering and yield formation stages. A 50 m³ farm pond with a drip system irrigation system was found adequate to meet supplemental irrigation requirement for a kitchen garden of 300–600 m² planted with a 90 days growing period cabbages. The cost-benefit analysis showed that farm ponds are feasible solutions to persistent crop failures in semi-arid areas which dominant most countries in Sub-Saharan Africa (SSA).     

Trickle and sprinkler irrigation of potato (Solanum tuberosum L.) in the Middle Anatolian Region in Turkey

The potato (Solanum tuberosum L.) is widely planted in the Middle Anatolian Region, especially in the Nigde-Nevsehir district where 25% of the total potato growing area is located and produces 44% of the total yield. In recent years, the farmers in the Nigde-Nevsehir district have been applying high amounts of nitrogen (N) fertilizers (sometimes more than 900 kg N ha⁻¹) and frequent irrigation at high rates in order to get a much higher yield. This situation results in increased irrigation and fertilization costs as well as polluted ground water resources and soil. Thus, it is critical to know the water and nitrogen requirements of the crop, as well as how to improve irrigation efficiency. Field experiments were conducted in the Nigde-Nevsehir (arid) region on a Fluvents (Entisols) soil to determine water and nitrogen requirements of potato crops under sprinkler and trickle irrigation methods. Irrigation treatments were based on Class A pan evaporation and nitrogen levels were formed with different nitrogen concentrations.The highest yield, averaging 47,505 kg ha⁻¹, was measured in sprinkler-irrigated plots at the 60 g m⁻³ nitrogen concentration level in the irrigation treatment with limited irrigation (480 mm). Statistically higher tuber yields were obtained at the 45 and 60 g m⁻³ nitrogen concentration levels in irrigation treatments with full and limited irrigation. Maximum yields were obtained with about 17% less water in the sprinkler method as compared to the trickle method (not statistically significant). On the loam and sandy loam soils, tuber yields were reduced by deficit irrigation corresponding to 70% and 74% of evapotranspiration in sprinkler and trickle irrigations, respectively. Water use of the potato crop ranged from 490 to 760 mm for sprinkler-irrigated plots and 565–830 mm for trickle-irrigated treatments. The highest water use efficiency (WUE) levels of 7.37 and 4.79 kg m⁻³ were obtained in sprinkle and trickle irrigated plots, respectively. There were inverse effects of irrigation and nitrogen levels on the WUE of the potato crops. Significant linear relationships were found between tuber yield and water use for both irrigation methods. Yield response factors were calculated at 1.05 for sprinkler methods and 0.68 for trickle methods. There were statistically significant linear and polynomial relationships between tuber yield and nitrogen amounts used in trickle and sprinkler-irrigated treatments, respectively. In sprinkler-irrigated treatments, the maximum tuber yield was obtained with 199 kg N ha⁻¹. The tuber cumulative nitrogen use efficiency (NUE_cu) and incremental nitrogen use efficiency (NUE_in) were affected quite differently by water, nitrogen levels and years. NUE_cu varied from 16 to 472 g kg⁻¹ and NUE_in varied from 75 to 1035 g kg⁻¹ depending on the irrigation method. In both years, the NH₄-N concentrations were lower than NO₃-N, and thus the removed nitrogen and nitrogen losses were found to be 19–87 kg ha⁻¹ for sprinkler methods and 25–89 kg ha⁻¹ for trickle methods. Nitrogen losses in sprinkler methods reached 76%, which were higher than losses in trickle methods.     

Effects of drip irrigation frequency on soil wetting pattern and potato growth in North China Plain

The effect of irrigation frequency on soil water distribution, potato root distribution, potato tuber yield and water use efficiency was studied in 2001 and 2002 field experiments. Treatments consisted of six different drip irrigation frequencies: N1 (once every day), N2 (once every 2 days), N3 (once every 3 days), N4 (once every 4 days), N6 (once every 6 days) and N8 (once every 8 days), with total drip irrigation water equal for the different frequencies. The results indicated that drip irrigation frequency did affect soil water distribution, depending on potato growing stage, soil depth and distance from the emitter. Under treatment N1, soil matric potential (ψ_m) Variations at depths of 70 and 90 cm showed a larger wetted soil range than was initially expected. Potato root growth was also affected by drip irrigation frequency to some extent: the higher the frequency, the higher was the root length density (RLD) in 0–60 cm soil layer and the lower was the root length density (RWD) in 0–10 cm soil layer. On the other hand, potato roots were not limited in wetted soil volume even when the crop was irrigated at the highest frequency. High frequency irrigation enhanced potato tuber growth and water use efficiency (WUE). Reducing irrigation frequency from N1 to N8 resulted in significant yield reductions by 33.4 and 29.1% in 2001 and 2002, respectively. For total ET, little difference was found among the different irrigation frequency treatments.     

Spatial and temporal distributions of nitrogen and crop yield as affected by nonuniformity of sprinkler fertigation

Deep percolation and nitrate leaching are important considerations in the design of sprinkler systems. Field experiments were therefore conducted to investigate the influence of nonuniformity of sprinkler irrigation on deep percolation and spatial distributions of nitrogen and crop yield during the growing season of winter wheat at an experiment station in Beijing, China. Three experimental plots of a sandy clay loam soil in the 0–40 cm depth interval and a loamy clay soil below 40 cm were irrigated with a sprinkler irrigation system that had a seasonal averaged Christiansen irrigation uniformity coefficient (CU) varying from 72 to 84%. Except for the fertilizer applied before planting, fertilizer was applied with the sprinkler irrigation system. The corresponding seasonal averaged CU for fertigation varied from 71 to 85%. Daily observation of matrix water potentials in the root zone showed that little deep percolation occurred. Consequently, the effect of sprinkler uniformity on deep percolation was minor during the irrigation season for the soil tested. Intensive gravimetric soil core samplings were conducted several times during the irrigation season in a grid of 5 m × 5 m for each plot to determine the spatial and temporal variation of NH₄-N and NO₃-N contents. Soil NH₄-N and NO₃-N exhibited high spatial variability in depth and time during the irrigation season with CU values ranging from 23 to 97% and the coefficient of variation ranging from 0.04 to 1.06. A higher uniformity of sprinkler fertigation produced a more uniform distribution of NH₄-N, but the distribution of NO₃-N was not related to fertigation. Rather it was related to the spatial variability of NO₃-N before fertigation began. At harvest, the distribution of dry matter above ground, nitrogen uptake, and yield were measured and the results indicated that sprinkler fertigation uniformity had insignificant effects on the parameters mentioned above. Field experimental results obtained from this study suggest that sprinkler irrigation if properly managed can be used as an efficient and environment-friendly method of applying water and fertilizers.     

Design,construction and erection of seawater intake system to establish a biofouling test facility

Large amount of seawater is used in coastal industries, like power generating plants, desalination plants and aquatic culture farms. A similar requirement exists in Department of Atomic Energy campus at Kalpakkam, Tamil Nadu, India. Seawater in large quantities is required for its nuclear power stations and desalination plants erected on the east coast of Bay of Bengal situated in southern part of India. Such seawater systems have biofouling problems. Hence a seawater intake system has been designed, constructed and erected so that virgin seawater will be available for biofouling studies that was proposed to be carried in a once through flow test facility. A major criterion for the test facility was that continuous supply of seawater should be available at the rate of 160 m³/h. To meet this requirement, centrifugal pumps were installed at about 150 m away from the shoreline and connected to an intake structure using 600 m long, 355 mm OD, high density polyethylene pipeline laid on the seabed. Details of site selection, options of construction methods, materials selection, pressure drop calculations, sizing of pipes and anchor blocks, stability of intake pipeline, deployment criteria and project cost and planning have been discussed in this paper.     

Soil microarthropods are only weakly impacted after 13 years of repeated drought treatment in wet and dry heathland soils

Studies of biological responses in the terrestrial environment to rapid changes in climate have mostly been concerned with aboveground biota, whereas less is known of belowground organisms. The present study focuses on mites and springtails of heathland ecosystems and how the microarthropod community has responded to simulated climate change in a long-term field experiment. Increased temperature and repeated drought was applied for 13 years to field plots located in Wales, The Netherlands and Denmark representing sites of contrasting climatic conditions with respect to precipitation and temperature. This approach provided an opportunity to study biological responses on a local (within sites) and regional scale. Warming treatments increasing night time temperature (0.3–1 °C higher than ambient at 5 cm soil depth) had no detectable effects on the microarthropod communities. Increased intensity and frequency of drought had only weak persistent effects on springtail species composition, but practically no effect on major mite groups (Oribatida, Prostigmata or Mesostigmata) suggesting that ecosystem functions of microarthropods may only be transiently impacted by repeated spring or summer drought.